using nDNA to infer human evolutionary history

From: Marcio Pie (pie@bu.edu)
Date: Wed Oct 11 2000 - 07:17:12 EDT

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    Hi, folks!

    This is a very interesting paper using nuclear DNA to infer past history of
    human evolution that just came out in PNAS. This is the abstract.

    Peace

    Marcio

    Worldwide DNA sequence variation in a 10-kilobase noncoding region on human
    chromosome 22

    Zhongming Zhao*, Li Jin*, Yun-Xin Fu*, Michele Ramsay, Trefer Jenkins,
    Elina Leskinen, Pekka Pamilo, Maria Trexler§, Laszlo Patthy§, Lynn B.
    Jorde¶, Sebastian Ramos-Onsins*, Ning Yu, and Wen-Hsiung Li,**

    PNAS Vol. 97, Issue 21

    Human DNA sequence variation data are useful for studying the origin,
    evolution, and demographic history of modern humans and the mechanisms of
    maintenance of genetic variability in human populations, and for detecting
    linkage association of disease. Here, we report worldwide variation data
    from a 10-kilobase noncoding autosomal region. We identified 75 variant
    sites in 64 humans (128 sequences) and 463 variant sites among the human,
    chimpanzee, and orangutan sequences. Statistical tests suggested that the
    region is selectively neutral. The average nucleotide diversity () across
    the region was 0.088% among all of the human sequences obtained, 0.085%
    among African sequences, and 0.082% among non-African sequences, supporting
    the view of a low nucleotide diversity (0.1%) in humans. The
    comparable value in non-Africans to that in Africans indicates no severe
    bottleneck during the evolution of modern non-Africans; however, the
    possibility of a mild bottleneck cannot be excluded because non-Africans
    showed considerably fewer variants than Africans. The present and two
    previous large data sets all show a strong excess of low frequency variants
    in comparison to that expected from an equilibrium population, indicating a
    relatively recent population expansion. The mutation rate was estimated to
    be 1.15 × 109 per nucleotide per year. Estimates of the long-term effective
    population size Ne by various statistical methods were similar to those in
    other studies. The age of the most recent common ancestor was estimated to
    be 1.29 million years ago among all of the sequences obtained and 634,000
    years ago among the non-African sequences, providing the first evidence
    from a noncoding autosomal region for ancient human histories, even among
    non-Africans.



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