New genetic data indicates mankind's ancestry >1 million years

From: Glenn Morton (glenn.morton@btinternet.com)
Date: Sun Feb 18 2001 - 11:41:29 EST

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    Over the past few months genetic data has been accumulating which will
    seriously upset the preferred apologetical schemes. This data is clearly
    indicating that humanity has genetically subdivided for over a million
    years. By this I mean that to account for the polymorphism of some genes, we
    must posit this length of time for the mutations to occur. For the old earth
    creationist this means that the concept that Adam was a recent creation is
    no longer tenable and for the young-earth creationist who believes in low
    mutation rates, the genetic data provides an independent dating for a
    history of human kind much longer than 6000 years. A commentary in the
    Proc. Natl. Acad. Sci. USA, Jan 30, 2001 had some very interesting things to
    say about the current status of genetics as it relates to human history.

            “It has been an interesting decade in Human evolutionary genetics. We have
    gone from hope to confidence and from confidence to despair. A series of
    recent studies, two of which appear in this and a recent issue of PNAS,
    gives new grounds for optimism.” Alan R. Rogers, “Order Emerging from Chaos
    in Human Evolutionary Genetics,” PNAS, 93(2001):3:779-780, P. 779

    Rogers then goes on to say:

    “Various authors argued early on that the human population must have passed
    through a bottleneck—a period of small population size—sometime during the
    late Pleistocene.
            “The next few years saw improvements both in the quality of mitochondrial
    data available and in the statistical methods available for dealing with
    those data. By the mid-1990s, we were able not only to reject the hypothesis
    of stationary population size but also to place an upper bound on the
    preexpansion population size, a lower bound on the postexpansion size, and
    both bounds on the time of the expansion. The evidence indicated that,
    sometime between 30,000 and 130,000 years ago, our ancestors expanded to
    fill the globe from an initial population of roughly 10,000 breeding
    individuals. Then just as this story seemed to be gaining momentum, the
    bottom fell out from under it.
            “In the late 1990s, as DNA sequencing got cheaper, people began assembling
    large datasets from the human nuclear genome. The trouble was that each
    genetic locus seemed to tell a different story. The mitochondrial story
    received support from data from the Y chromosome and from extensive sets of
    short tandem repeat (STR) loci distributed throughout the genome. But other
    genes seemed to imply a long history of constant population size, and still
    others suggested the action of balancing selection or of geographic
    population structure. How can a single species have genes with such
    disparate histories? Presumably because natural selection has affected
    different loci in different ways. And if the pattern we see is telling us
    mainly about the history of selection, then it is unlikely ever to tell us
    much about the history of population size.
            “This was not, of course, the first time that anyone had suggested a role
    for natural selection in the evolution of human mitochondria. The problem is
    that selection and population growth can be hard to tell apart. A favorable
    mutation may sweep through the population under the influence of natural
    selection. If we focus on the carriers of this favorable mutation, the
    process looks just like population growth: the number of carriers is small
    at first, then increases, and then levels off. For practical purposes, the
    two processes have identical effects on genetic variation. There is still no
    clean way of distinguishing them except by comparing DNA from different
    genetic loci. Population growth should affect every locus in the same way,
    whereas selection should affect different loci in different ways. The
    disparate results that we see from different loci’ suggest that human
    genetic variation is influenced strongly by natural selection. If this view
    is correct, genetics may have little to tell us about population history.
            “This view, however may be unnecessarily gloomy. There are two aspects of
    the emerging pattern that are puzzling under the view that it is all a
    product of natural selection: First, the genetic loci that show the
    signature of a selective sweep are precisely the ones that, on a priori
    grounds, seem most likely to be neutral—the sweeps all seem to have occurred
    in DNA that does not code for protein. It is coding only regions that seem
    clearly consistent with selective neutrality and constant population size.
    This pattern is not as implausible as it may sound: Because of linkage, the
    signature of a selective sweep may extend from the coding region on which
    selection has acted into the noncoding regions that surround it.
    Nonetheless, it is surprising that the effects of selection should be most
    clearly visible in those portions of the genome that do not code for
    protein. It is also hard to imagine that selection at linked loci was
    responsible for the pattern seen in the STRs, because these are distributed
    widely throughout the genome.” Alan R. Rogers, “Order Emerging from Chaos in
    Human Evolutionary Genetics,” PNAS, 93(2001):3:779-780, P. 779

    The article Rogers refers to says that the coalescence time for the gene
    system they studied is quite long ago. The gene system was the ms205
    minisatellite. They say of the variation seen among Basques, Japanese,
    British and Africans:

    “The coalescent adds a time dimension to the phylogenetic network (tree);
    thus, assuming neutrality, panmixia, and constancy in population size, the
    depth of the tree (the time to the most recent common ancestor) is estimated
    as 0.72 coalescent units, or about 1.04 million years (…).” Santos Alonso
    and John A. L. Armour, “A Highly Variable Segment of Human Subterminal 16p
    Reveals a History of Population Growth for Modern Humans Outside Africa,”
    Proc. Natl. Acad. Sci., USA, 98(2001):3:864-869, p. 868.

    One million years ago, the only hominid on earth was H. erectus. We have his
    genes, which means we interbred and are descended from him. Indeed, even
    more important is the FACT now, that there were separate lineages over that
    time span which are found in modern humans. We were not, as many Christian
    apologists contend, the sudden creation de novo less than 120,000 years ago.

    Indeed, there is evidence that non-Africans have a genetic history going
    back at least 600,000 years—much longer ago than the Out of Africa view
    would allow. Consider a paper published last year which said:

    "Human DNA sequence variation data are useful for studying the origin,
    evolution, and demographic history of modern humans and the mechanisms of
    maintenance of genetic variability in human populations, and for detecting
    linkage association of disease. Here, we report worldwide variation data
    from a 10-kilobase noncoding autosomal region. We identified 75 variant
    sites in 64 humans (128 sequences) and 463 variant sites among the human,
    chimpanzee, and orangutan sequences. Statistical tests suggested that the
    region is selectively neutral. The average nucleotide diversity ( p [pi])
    across the region was 0.088% among all of the human sequences obtained,
    0.085% among African sequences, and 0.082% among non-African sequences,
    supporting the view of a low nucleotide diversity (0.1%) in humans. The
    comparable p [pi] value in non-Africans to that in Africans indicates no
    severe bottleneck during the evolution of modern non-Africans; however, the
    possibility of a mild bottleneck cannot be excluded because non-Africans
    showed considerably fewer variants than Africans. The present and two
    previous large data sets all show a strong excess of low frequency variants
    in comparison to that expected from an equilibrium population, indicating a
    relatively recent population expansion. The mutation rate was estimated to
    be
    1.15 × 10^9 per nucleotide per year. Estimates of the long-term effective
    population size Ne by various statistical methods were similar to those in
    other studies. The age of the most recent common ancestor was estimated to
    be 1.29 million years ago among all of the sequences obtained and 634,000
    years ago among the non-African sequences, providing the first evidence from
    a noncoding autosomal region for ancient human histories, even among
    non-Africans. Zhongming Zhao, et al, “Worldwide DNA sequence variation in a
    10-kilobase noncoding region on human chromosome 22” Proc. Natl. Acad. Sci.
    USA, Vol. 97, Issue 21, 11354-11358, October 10, 2000
    http://www.pnas.org/cgi/content/abstract/97/21/11354

    This article gives the time to the most recent common ancestors in Table 5
    (reproduced below). Note that the 95% confidence interval on both sides of
    the mean are ALL PRIOR TO THE TIME THAT ANATOMICALLY MODERN MAN WAS ON
    EARTH. This data demonstrates the high likelihood of independent genetic
    lineages stretching back long before the Out of Africa view would allow. And
    it is the Out of Africa view that many Christian apologists like Ross,
    Newman, Weister and Wilcox have incorporated into their apologetical
    schemes. And they are wrong. Here is table 5 — (Ne is the effective
    breeding population)

    “Table 5. The age (T, 10^3 years) of the MRCA of human sequences

    Sequences Ne Tmode Tmean 95% Interval

    All samples 10,000 1,288 1,356 712~2,112
                         12,000 1,104 1,203 605~1,949
                       15,000 924 1,034 504~1,728
    Africans 6,000 1,204 1,256 694~1,882
                        8,000 1,158 1,203 646~1,843
                       10,000 1,032 1,105 576~1,752
    Non-Africans 6,000 747 806 384~1,330
                        7,000 678 756 353~1,277
                        8,000 634 713 333~1,229

    The average mutation rate (2.28 × 10^4 per sequence per generation) was
    used. “ source Zhongming Zhao, et al, “Worldwide DNA sequence variation in a
    10-kilobase noncoding region on human chromosome 22” Proc. Natl. Acad. Sci.
    USA, Vol. 97, Issue 21, 11354-11358, October 10, 2000

    Note above that the upper limits of the 95% confidence interval incorporates
    the earliest H. erecti.

    The paper says:
    “The TMRCA [The Most Recent Common Ancestor--grm] of Africans was about 0.4
    Myr older than that of non-Africans (1,032,000 versus 634,000) (Table 5).
    However, the TMRCA of non-Africans from our data are older than the fossils
    for modern humans (see ref. 3) and is also older than those of non-Africans
    estimated from PDHA1, the ß[beta]-globin gene, and mitochondrial DNA (1, 3,
    8, 27). It indicates that even non-Africans have ancient genetic histories,
    at least at some regions. Of course, a substantial or large part of the
    genetic diversity in non-Africans at the region we studied might be due to
    migration from Africa. However, the genealogical depths at this region and
    the ß[beta]-globin gene region (1) in non-Africans and the long separation
    time between African and non-African sequences at the PDHA1 region (3)
    suggest that the transformation from archaic to modern humans might have
    occurred in a subdivided population. This does not contradict the assumption
    of the African origin of modern humans and also does not imply independent
    evolution of modern characteristics in separate populations as implied by
    the multiregional model, because the transformation could have occurred
    through gene flow and natural selection (3). However, it does suggest that
    the origin and evolution of modern humans is more complex than depicted by
    the simple out of Africa model (1, 3), especially the assumption of a
    complete replacement of all indigenous populations outside of Africa by the
    African stock. “Zhongming Zhao, et al, “Worldwide DNA sequence variation in
    a 10-kilobase noncoding region on human chromosome 22” Proc. Natl. Acad.
    Sci. USA, Vol. 97, Issue 21, 11354-11358, October 10, 2000

    A note that unfortunately must be said in this day of racial sensitivity.
    One can not use this data to say that the races were genetically distinct.
    We aren't. The data accumulated by Alonso and Armour studied the DNA of 50
    people, meaning that here were 100 individual strands of nuclear DNA
    sequenced. The most popular allele (48) was shared by 13 Basques, 11
    Japanese, 14 Brits, 8 Kenyans and 2 Pygmies (see p. 865 of Alonso and
    Armour). Among other ethnically shared lineages are one each for the Basque
    and Japanese; the Basques, Brits and Kenyans; the Basques and the Kenyans;
    the Basques Japanese, Kenyans and Pygmies; and the Pygmies and Kenyans. That
    being said, each of the populations sampled contained several unique
    sequences. The pygmies had 8 unique lineages; the Japanese, 3; the Brits, 4;
    the Basques, 1; and the Kenyans, 4. Clearly, while the unique genetic
    lineages indicate a long time span back to the most recent common ancestor,
    the lineages shared by various populations are widely enough shared to know
    that we humans are clearly one interbreeding population and have been for
    the past 1 million years.

    These data fits well with other recent studies, such as the fact that
    anatomically modern Mungo Man has an archaic form of mtDNA requiring
    breeding with the archaics. [Proc. Natl. Acad. Sci. USA 98(2001):537-542]

    These data fits well with the recent work by Wolpoff et al in which they
    showed that early European anatomically modern people shared more traits
    with Neanderthal than with the supposed African invaders [Milford H.
    Wolpoff, John Hawks, David W. Frayer, and Keith Hunley, “Modern Human
    Ancestry at the Peripheries: A Test of the Replacement Theory,” Science
    291(2001):293-297]

    It fits well with the discovery of a putative (but contested)
    Neanderthal-human hybrid child at Lagar Velho [Cidália Duarte et al, "The
    early Upper Paleolithic human skeleton from the Abrigo do Lagar Velho
    (Portugal) and Modern Human Emergence in Iberia " Proc. Natl. Acad.
    Sciences, USA, Vol. 96, Issue 13, 7604-7609; Erik Trinkaus, and Cidalia
    Duarte, “The Hybrid Child from Portugal,” Scientific American April 2000, p.
    102]

    It fits well with the data of Wallace et al in which the mutation that may
    cause Alzheimer's disease was inserted into the human genome 770,000 years
    ago. [Douglas C. Wallace, et al, “Ancient mtDNA sequences in the Human
    Nuclear Genome: A Potential Source of Errors in Identifying Pathogenic
    Mutations,” Proc. Natl. Acad. Sci., 94(1997):14900-14905, p. 14900]

    In my opinion, Christians are tying themselves to a sinking ship by placing
    such apologetical emphasis on the supposed recent (<120,000 year) creation
    of man. There are two reasons for this belief. First, the new genetic
    evidence is changing the landscape. It is changing the viability of the most
    popular Christian view of human origins and it is providing a means of
    dating the very creation of man which is also inconsistent with the other
    popular view, the YEC view (already falsified many times over but what is
    one more falsification between friends?). Secondly, Christians have tied
    themselves with the epistemologically weakest view of human origins. It has
    long been known that if the time to the most recent common mitochondrial
    ancestor was around 200,000 years, then the time to the most recent nuclear
    genomic ancestor would be 4-9 times that long. Christian apologists have
    long ignored the warning about this that I have been giving for the past
    several years (http://www.calvin.edu/archive/asa/199806/0083.html).
    Templeton states:

    "If the coalescence time of mtDNA is truly about 200,000 years ago, then the
    expected coalescence time of almost all nuclear genes are going to be
    commonly greater than one or two million years. This places the expected
    coalescence times of much nuclear DNA into a period in which all humans
    probably lived in Africa. Hence, studies on nuclear DNA are expected to
    have an African root under all hypotheses of modern human evolution." ~ Alan
    R. Templeton, "Testing the Out of Africa Replacement Hypothesis with
    Mitochondrial DNA Data," in G. A. Clark and C. M. Willermet, ed., Conceptual
    Issues in Modern Human Origins Research, (New York: Aldine de Gryuter,
    1997), pp. 329-360, p. 353

    In order for the recent origin of man, as envisioned by Christian
    apologists, to be true, ALL GENETIC SYSTEMS MUST BE LESS THAN THAT AGE. That
    means that no genes can have coalescence times of greater than 200,000
    years. This clearly is falsified by the new data (and we should have seen it
    coming). Christian apologists have tied themselves to the mitochondrial data
    hoping that it would win the day for them. Unfortunately, to falsify this
    position required only one genetic system be found with a coalescence
    time longer than 200,000 years, which we now have. Obviously the concept of
    a recent origin of man is less defendable than the ancient origin of man.

    One further implication of this data must be noted. Either the Bible is
    totally false in what it says about Adam or one must do what I am
    doing--extend the Biblical chronology. To claim, in the face of this
    evidence, that modern man was a de novo creation within the past 200,000
    years is to do the same thing the young-earth creationists do--ignore the
    data and believe what we want to believe regardless of the observational
    data.

    glenn

    see http://www.glenn.morton.btinternet.co.uk/dmd.htm
    for lots of creation/evolution information
    anthropology/geology/paleontology/theology\
    personal stories of struggle



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